Potential of Ulva lactuta Crude Extract in Increasing Total Haemocyte Count (THC) and Fagocytic Activity in Vaname Shrimp (Litopenaeus vannamei)

Suleman Suleman; Sri Andayani; Ating Yuniarti

doi:10.35316/jsapi.v10i1.230

Suleman Suleman Mahasiswa Magister Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, Malang
Sri Andayani Dosen Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, Malang
Ating Yuniarti Dosen Fakultas Perikanan dan Ilmu Kelautan Universitas Brawijaya, Malang

10.35316/jsapi.v10i1.230

Keywords: Immunostimulant, Crude extract, vaname shrimp, Ulva lactuta.

Abstract

Acauses of the decrease in vaname shrimp production was vibriosis. Vibriosis is caused by vibrio bacteria. A vibrio bacteria that plays a role in vibriosis is vibrio harveyi. Managing of diseases caused by vibrio harveyi is one of the best that provides immunostimulant assistance from natural ingredients derived from seaweed. Ulva lactuta is a green seaweed that has different substances that contain immune. Some of the compositions contained in Ulva Lactutawere Flavonoid Compositions, and the Composition of Polysaccharides that are Combined with Immunimulants The purpose of this study was to determine the effect and dosage used to improve the immune system in vaname shrimp. Combined with THC and Fagocytosis in the hemolim on vaname shrimp before infection and after infection with Vibrio harveyi bacteria.A dose of 2 ppm showed a significant difference (p <0.05) for all treatments.

Downloads

Download data is not yet available.

References

Austin and Austin. 2007. Bacterial Fish Pathogen. Diseases of Farmed and Wild Fish. Springer. Fourth Edition

Castro, R.I. Zarrab, and J. Lamas. 2004. Water-soluble Seaweed Extracts Modulate the Pantoea agglomerans lippopoly saccaharide (LPS). Fish Shellfish Immunology, 10:555-558

Cheng, W. Wang, L and J. Chen. 2005. Effect of water temperature on the immune response of white shrimp Litopenaeus vannamei to vibrio alginolyticus. Aquaculture, 250:592-601

Effendy, S. Alexander, R dan T. Akbar. 2004. Peningkatan hemosit benur udang windu (Penaeus monodon Fab) pasca perendaman ekstrak ragi roti (Saccharmoyces cerevisiae) pada konsentrasi yang berbeda. Jurnal Sains dan Teknologi, 14(2):46-53

Ermantianingrum, A.A., Sari, R., dan S.B. Prayitno. 2013. Potensi Chlorella sp. Sebagai Imunostimulan untuk Pencegahan Penyakit Bercak Putih (White Spot Syndrome Virus) pada Udang Windu (Penaeus monodon). Journal of Aquaculture Management and Technology. Vol.1(1),206-221.

Febriani, D., Sukenda dan S.Nuryati. 2013. Kappa-Karagenan sebagai imunostimulan untuk pengendalian Penyakit Infectious myonecrosis (IMN) pada udang vaname (Litopenaeus vannamei). Jurnal Akuakultur Indonesia. 12(1):70-78.

Ghaednia, B. Mehrabi M. R. Mirkbaksh, M. Yeganeh, V. Hoseinkhezri. Garibi, G and J. A .Gahffar. 2011. Effect of Hot Water Extract of Brown Seaweed Sargassum glaucescens via Immersion route on Immune Responses of Fenneropenaeus indicus. Iranian Journal of Fisheries Sciences. 10:616-630

Hatmanti, A. 2003. Penyakit Bakterial Pada Budidaya Krustasea serta Cara Penanganannya. Oceana. Vol. XXVIII, No.3:1-10

Jasmindar, Y. 2009. Penggunaan Ekstrak Gracillaria verrucosa untuk meningkatkan system ketahanan Udang vaname Litopenaeus vannamei. Bogor. Institut Pertanian Bogor.

Johansson, M.W., Keyser, P., Sritunyalucksana, and K. Soderhall. 2000. Crustacean Haemocytes and Haematopoiesis. Aquaculture.191:45-52.

Liu, C.H and J.C. Chen. 2004. Effect of Ammonia on the Immune response of White shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus. Fish & Shellfish Immunology. 16: 321-334

Maftuch. Toban, M. H and Y. Risjani. 2012. Administration of marine algae (Gracilaria verrucosa) immunostimulant enhances some innate immune parameters in black tiger shrimp (Penaeus monodon Fabricus) against Vibrio harveyi infection. Journal of Applied Research. 8(2):1052-1058

Nitimulyo, K. H., A. Isnanstyo, Triyanto, I. Istiqomah dan M. Murdjani. 2005. Isolasi, Identifikasi dan Karakterisasi Vibrio spp. Patogen Penyebab vibriosis pada Kerapu di Balai Budidaya Air Payau Situbondo. Jurnal Perikanan. Vol VII (2):80-94

Paulert, R. Junior, A.S. Stadnik, M.J and M.G. Pizzolatti. 2007. Antimikrobial properties of extract from the green seaweed Ulva fasciata Delile against pathogenic bacteria and fungi. Algological Studies. 123-130

Pope, E.C, Powell, A., Roberts, E.C., Shields, R.J., Wardle, R., and A.F. Rowley. 2011. Enhanced Cellular immunity in Shrimp (Litopenaeus vannamei) after ‘vaccination’. PLos ONE 6.

Ridlo, A dan R. Pramesti. 2009. Aplikasi ekstrak rumput laut sebagai agen immunostimulan sistem pertahanan non spesifik pada udang (Litopenaeus vannamei). Ilmu Kelautan. Vol.14:133-137

Rodrigues,J. and G. L. Moullac. 2000. State of the art if immunological tools and health kontrol of penaeid shrimp. Aquaculture.191:109-119

Sarjito, Apriliani,M. Afriani dan D. A. C. Haditomo. 2015. Agensia Penyebab Vibriosis Pada Udang Vanname (Litopenaeus gariepinus) yang Dibudidayakan secara Intensif di Kendal. Jurnal Kelautan Tropis. Vol 18(3):189-196

Simamora, S.D. 2014. Market Brief Langkah dan Strategi Ekspor ke uni Eropa:Produk Udang. Advancing Indonesia’s Civil Society in Trade and Investment Climate. APINDO

Supamattaya, K., Kiriratnikom, S., Boonyaratopalin, M. and L. Borowitzka. 2005. Effect of a Dunaliella Extract on Growth Performance, Health Condition, Immune Response and Disease resistance in Black Tiger Shrimp (Penaeus monodon). Aquaculture. 248(1):207-216

Van de Braak K. 2002. Haemocytic Defence in Black Tiger Shrimp (Penaeus monodon). Thesis. Wageningen. Belanda. Hal.159

Xu D., Liu, W., Alvarez A. and T.Huang. Cellular Immune responses against viral Pathogens in Shrimp. Developmental and Comparative Immunology. 47:287-297.