Potential Antivirus Viral Nervous Necrosis Methanol extract of Amphora sp. in Cantang Grouper (Epinephelus sp.)

Ach. Khumaidi; Astik Umiyah

doi:10.35316/jsapi.v10i2.538

Ach. Khumaidi Fakultas Sains dan Teknologi Universitas Ibrahimy
Astik Umiyah 2) Departement Kebidanan, Fakultas Ilmu Kesehatan, Universitas Ibrahimy, Situbondo.

10.35316/jsapi.v10i2.538

Keywords: hybrid grouper, diatoms, VNN, aquaculture, disease.

Abstract

Cantang grouper received more serious attention from grouper fish farmers due to high export interest, but the attack of viral nervous necrosis (VNN) became a major obstacle in its cultivation. This study aims to explore the antiviral potential of diatom Amphora sp. to counter the VNN attack on Cantang groupers. The method used is the extraction of Amphora sp. with absolute methanol solvent. The extraction results were tested in vivo by giving Amphora sp. with different concentrations, namely: 17 µg / ml, 33 μg / ml, 50 μg / ml, and administration of extracts by challenging the 17 μg / ml + VNN, 33 μg / ml + VNN, 50 μg / ml + VNN. Fish treated with Amphora sp. also challenged by giving VNN Positive fish meat. During the period of rearing fish observed clinical behavior and symptoms. After the fish were raised for 15 days, the fish harvested were analyzed using histology, RT-PCR, and CPI methods (using the immuno ratio software) to see the HSP immune response obtained from the administration of Amphora sp. to find out its potential as a natural antivirus. From several test parameters, the concentration of extract 50 mg / ml + VNN was given to give the best response in the CPI analysis with a DAB value (61.3%). These results indicate that the methanol extract of Amphora sp. has the potential to be used as an antiviral candidate in Cantang grouper fish.

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References

Boukhris, Saoussan, Khaled Athmouni, Ibtissem Hamza-Mnif, Rayda Siala-Elleuch, Habib Ayadi, Moncef Nasri, and Alya Sellami-Kamoun. 2017. “The Potential of a Brown Microalga Cultivated in High Salt Medium for the Production of High-Value Compounds.” BioMed Research International 2017.
Deane, Eddie E., Norman Y. S. Woo, E. Eddie, and Norman Y. S. Woo. 2019. “Differential Gene Expression Associated with Euryhalinity in Sea Bream ( Sparus Sarba ).” American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 287(5):1054–63.
Heo, Soo-jin and You-jin Jeon. 2009. “Journal of Photochemistry and Photobiology B : Biology Protective Effect of Fucoxanthin Isolated from Sargassum Siliquastrum on UV-B Induced Cell Damage C3-1 C3-2 C3-3.” Journal of Photochemistry & Photobiology, B: Biology 95(2):101–7.
Heo, Soo-Jin, Seok-Chun Ko, Sung-Myung Kang, Hahk-Soo Kang, Jong-Pyung Kim, Soo-Hyun Kim, Ki-Wan Lee, Man-Gi Cho, and You-Jin Jeon. 2008. “Cytoprotective Effect of Fucoxanthin Isolated from Brown Algae Sargassum Siliquastrum against H 2 O 2 -Induced Cell Damage.” European Food Research and Technology 228:145–51.
Katayama, Tomoyo, Ai Murata, and Satoru Taguchi. 2011. “Responses of Pigment Composition of the Marine Diatom Thalassiosira Weissflogii to Silicate Availability during Dark Survival and Recovery.” Plankton & Benthos Research 6(1):1–11.
Khumaidi, Ach, Mohamad Fadjar, Feni Iranawati, and Yuni Kilawati. 2019. “Mass Mortality Associated with Viral Nervous Necrosis of Hybrid Grouper ( Epinephelus Sp .) Cultured in City of Grouper Mass Mortality Associated with Viral Nervous Necrosis of Hybrid Grouper ( Epinephelus Sp .) Cultured in City of Grouper.” 070007(July).
Koesharyani, Isti and Hessy Novita. 2006. “Diagnosa ‘Nested Reverse Transcriptase - PCR’ Untuk ‘Viral Nervous Necrosis’ Pada Benih Ikan Kerapu Bebek, Cromileptes Altivelis [in Indonesian].” Jurnal Manusia Dan Lingkungan 1(3):382–86.
Mustopa, A. Zaenal, Dwi Susilaningsih, Linda Sukmarini, Muhammad Ridwan, Delicia, and Hasim. 2012. “Pengembangan Polisakarida Dari Mikroalga Btm 11 Sebagai Inhibitor Rna Helikase Virus Hepatitis C.” Prosiding InSINas.
Raihan Othman, Amni, Gunzo Kawamura, Shigeharu Senoo, and Ching Fui Fui. 2015. “Effects of Different Salinities on Growth, Feeding Performance and Plasma Cortisol Level in Hybrid TGGG (Tiger Grouper, Epinephelus Fuscoguttatusx Giant Grouper, Epinephelus Lanceolatus) Juveniles.” International Research Journal of Biological Sciences Int. Res. J. Biological Sci 4(3):2278–3202.
Sutarmat, Tatam and Tirta Yudha. 2013. “Analisis Keragaan Pertumbuhan Kerapu Hibrida Hasil Hibridisasi Kerapu Macan (Epinephelus Fuscoguttatus) Dengan Kerapu Kertang (Epinephelus Lanceolatus) Dan Kerapu Batik (Epinephelus Microdon) [in Indonesian].” Jurnal Riset Akuakultur 8(3):363–72.
Viera, M. P., Gomez. J. L. Pincehetti, G. C. Vicose, S. Suarez, J. Haroun, Ricardo, and M. S. Izquierdo. 2005. “Suitability of Three Red Macroalgae as a Feed for the Abalone Haliotis Tuberculata Coccinea Reeve Suitability of Three Red Macroalgae as a Feed for the Abalone Haliotis Tuberculata Coccinea Reeve.” Aquaculture 248(May 2014):75–78.
Yanuhar, U., I. Al-Hamidy, and N. R. Caesar. 2019. “Treatment of Chlorella Sp . Extract on Heat Shock Cluster (HSC) Response from the Tissue and Bloodcells Proliferation of Epinephelus Fuscoguttatus - Lanceolatus Infected by Viral Nervous Necrosis.” P. 012100 in IOP Conference Series: Earth and Environmental Science. Vol. 236.
Yuwanita, Rani and Uun Yanuhar. 2013. “Pathognomonic of Viral Nervous Necrotic ( VNN ) Virulence on Larvae of Humpback.” 7(6):1074–81.