INHIBITION OF Eucheuma spinosum EXTRACT WITH DIFFERENT CONCENTRATION OF Bacillus cereus

  • Madyasta Anggana Rarassari Program Studi Budidaya Perairan Fakultas Pertanian, Universitas Sriwijaya, Palembang
  • Darius Darius Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang
  • Hartati Kartikaningsih Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya, Malang
Keywords: Echeuma spinosum extract, Bacillus cereus, best concentration, bioactive compounds

Abstract

Eucheuma spinosum is one phylum of red algae, that can be used as a natural antibacterial. Indicator bacteria used were Bacillus cereus causes food spoilage. How to handle with antibotic, but the bacteria to become resistant. Therefore, it takes effort to get the antibacterial from natural material. The purpose of this study to get the best concentration of the Eucheuma spinosum extract to inhibit Bacillus cereus and obtain bioactive compounds contained in Eucheuma spinosum. Treatment concentration of 400 ppm, 800 ppm and 1600 ppm with 4 replications.Analysis of the test data using a completely randomized design factorial with a differentiator (Test F). The results produced the best antibacterial from Eucheuma spinosum extract with a concentration of 800 ppm. This is indicated by the average diameter of inhibition zone against Bacillus cereus in Eucheuma spinosum extract with a concentration of 800 ppm is 1,73 mm. While the results of the analysis by GC-MS methods allegedly contained 9 antibacterial compounds extracted from Eucheuma spinosum, such as Phenol, Eugenol, Pentadecane, Heptadecane, 3- Eicosene, 9- Eicosene, 1- Hexadecane, Docosane dan 1- Octadecene.

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References

Ballantine, D. L., W. H. Gerwick and S. M. Velez. 1987. Antibiotic Activity of Lipid Soluble Extract From Carribean Marine Algae. Hydrobiologia 151/ 152; 463-469.

Davis, S. 2000. Antibacterial Activity Of Extracts Of Six Macroalgae From The Northeastern Brazilian Coast. Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte; Departamento de Biologia, Universidade Federal do Ceara, Fortaleza, CE, Brasil. Brazilian Journal Of Microbiology. 33:311-313.

Harborne, J. B. 1987. Fitokimia.Penuntun Cara Modern Menganalisis Tumbuhan.ITB.Bnadung.

Johannes S.P. 2013. Uji Daya Inhibisi ekstrak kasar Flavonoid Sambiloto (Andrographis paniculasa) dan Temu Putih (Curcuma zedoaria Roscoe) Terhadap Aktivitas Tirosin Kinase Secara In Vitro.Institut Pertanian Bogor.

Kusini., Melki., Wike, A. 2013. Uji Antibakteri Ekstrak Gracillia sp (Rumput Laut) terhadap bakteri Eschercia coli dan staphylococcus aureus.Universitas Sriwijaya.

Kusumaningtyas, E., Lusi S., Estie A. 2008. Penentuan Golongan Bercak Senyawa Aktif Ekstrak n-heksan Alpinia galanga terhadap Candida albicans dengan Bioautografi dan Kromatografi Lapis Tipis. JITV Vol. 13 No. 4 Th. 2008

Lampe MF, Ballweber LM, Isaacs CE, Patton DL, Stamm WE. 1998. Killing of Chlamydia trachomatis by novel antimicrobial lipids adapted from compounds in human breast milk. Antimicro.Agents Chemo., 45: 1239-1244.

Munawaroh S. dan Handayani P.A. 2010.Ekstraksi Minyak Daun Jeruk Purut (Citrus hystrix D. C) dengan Pelarut Etanol dan N-heksana.Jurnal Kompetisi Teknik yol.2, No. 1.73-79.

Prayitno, A. 2006.Pengendalian Penyakit Vibrio harveyi dengan Ekstrak Rumput Laut pada Udang Windu (Penenus monodon Fab).PL-13.Disertasi.Program Pascasarjana Universitas Brawijaya Malang.

Pranowo, A.B. 2009. Senyawa Antibakteri Golongan Flavonoid Dari Buah Belimbing Manis (Averrhoa carambola LinnL). Universitas Udayana, Bukit Jimbaran. Bali.

Riniatsih, I dan Wilis A, R. 2009.Bioaktivitas Ekstrak dan Serbuk Lamun Enhalus acoroides dan Thalassia hemprichii pada vibrio alginolyticus dan Vibrio harveyii.Vol. 14 (3): 138 – 141.

Suptijah, 2003.Rumput Laut: Prospek dan Tantangannya. Falsafah Sains - Program Pasca Sarjana, Institut Pertanian Bogor. Bogor.

Srivastava, J.N., Kumar V. 2011.Antibacterial Activity of Crude Extracts of Spirulina Platensis and its Structural Elucidation of Bioactive Compound.Departement of Botany.Dayalbagh. India.

Wattimenna, J. R., Nelly, C., Sugiarso, Widanto, M. A., Sukandar, E. Y., Soemardji, A.A dan Setiad, A. R. 1991.Farmakodinamika dan Terapi Antibiotik.Gadjah Mada University Press.Yogyakarta.168 hlm.

Wikipedia. 2012. Bacillus cereus. http:id.wikipedia.org/wiki/Bacillus-cereus.

Wiyanto, D. B. 2010. Uji Aktivitas Antibakteri Ekstrak Rumput Laut Kappaphycus alvarezii dan Eucheuma denticullatum Terhadap Bakteri Aeromonas hydrophila dan Vibrio harveyi.[Disertasi] Program Pascasarjana Fakultas Perikanan Dan Ilmu Kelautan Brawijaya. Malang.

Published
2016-04-18
How to Cite
Rarassari, M. A., Darius, D., & Kartikaningsih, H. (2016). INHIBITION OF Eucheuma spinosum EXTRACT WITH DIFFERENT CONCENTRATION OF Bacillus cereus. Samakia : Jurnal Ilmu Perikanan, 7(1), 05-11. https://doi.org/10.5281/jsapi.v7i1.297
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