IDENTIFICATION AND HISTOPATHOLOGY Flavobacterium columnare ON TILAPIA (Oreochromis niloticus)

  • Andi Jumria Balai Karantina Ikan, Pengendalian Mutu dan Hasil Perikanan Kelas I Surabaya I
  • Novia Christi Prihartini 1Balai Karantina Ikan, Pengendalian Mutu dan Hasil Perikanan Kelas I Surabaya I
  • Alfiyah Alfiyah 1Balai Karantina Ikan, Pengendalian Mutu dan Hasil Perikanan Kelas I Surabaya I
Keywords: Columnaris disease, Flavobacterium columnare, Tilapia (Oreochromis niloticus), histopathology

Abstract

Flavobacterium columnare has been isolated from the cultivation of tilapia. Clinically, this bacterial infection is characterized by the occurrence of whitish gray changes in some parts of the head, around the mouth, gills, fins or body. The disease often ends in death, which causes great economic losses in the aquaculture industry. Of the 15 samples taken, only 5 samples showed clinical characteristics that were chronic. After tested by conventional method, only 3 samples showed F.columnare characteristic with bacterial colonies of pale yellow bacteria isolate with rhizoid edges, small size, long stem, gram negative and motile. The results of histopathologic testing of muscle organs, fins and gills, showed the presence of bacterial colonies suspected of F. colloid in muscle and also some necrosis points. In the kidney there are many macrophages and infiltration of inflammatory cells, while on the fins seen the existence of congestion and inflammatory cells. The gill damage found was hyperplasia that developed into fusion, congestion and necrosis.

Downloads

Download data is not yet available.

References

Afrianto, E dan E. Liviawaty.1992. Pengendalian Hama dan Penyakit Ikan.Kanisius.Yogyakarta. Hal 20-21.

Austin B. and Austin D. A. 1999.Bacterial Fish Pathogens: Disease of Farmed and Wild Fish, Heriot-Watt University, Edinburgh, UK.

Eissa, A.E., Zaki M.M and Aziz, A.A., 2010. Flavobacterium columnare/Myxobolus tilapiae Concurrent Infection in the Earthen Pond Reared Nile Tilapia (Oreochromis niloticus) during the Early Summer. Interdisc.Bio.Centr, 2 (5) : 1-10.

Bernardet J.F, Bowman J.P: The genus Flavobacterium. 2006. In The Prokaryotes: AHandbook on the Biology of Bacteria: Volume 7: Proteobacteria: Delta and Epsilon Subclasses. Deeply Rooting Bacteria. Edited by Dworkin M, Falkow S. New York: Springer Science+Business Media, LLC. p.481–531.

Bernardet, J.F. and Grimont, PAD., 1989. Deoxyribonucleic acid relatedness and phenotypic characterization of Flexibacter columnaris sp. nov., nom. rev.,Flexibacter psychrofilus sp. nov., nom. ver., and Flexibacter maritimus. Int. J. Syst. Bacteriol. 39 (3): 346-354.

Dalsgaard I. 1993.Virulence mechanisms in Cytophaga psychrophila and other Cytophaga-like bacteria pathogenic for fish.Ann Rev Fish Dis 3:127–144.

Decostere, A., Haesebrouck, F. and Devriese, L.A. 1997. Shieh Medium Supplemented with Tobramycin for Selective Isolation of Flavobacterium columnare(Flexibacter columnaris) from Diseased Fish. Journal of Clinical Microbiology, 35: 322-324.

Decostere A, Haesebrouck F, Devriese LA. 1998. Characterization of four Flavobacterium columnare (Flexibacter columnaris) strains isolated from tropical fish. Vet Microbiol. 62:35–45.

Decostere A. 2002. Flavobacterium columnare infections in fish: the agent and its adhesion to the gill tissue. Verhandelingen - KAGB 64:421–430.

Durborow, R. M., Thune R. L., Hawke J. P., and Camus A. C. 1998. Columnaris disease: a bacterial infection caused by Flavobacterium columnare. Publication 479, Aquaculture Center, Stoneville, Mich, USA.

Ferguson ,H.W. 2006. Systemic Pathology of Fish.2nd edition. London: Scottian Press.

Foscarini R. 1989. Induction and development of bacterial gill disease in the eel (Anguilla japonica) experimentally infected with Flexibacter columnaris: pathological changes in the gill vascular structure and in cardiac performance. Aquaculture 78:1–20.

Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. and Williams, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. Williams & Wilkins, Baltimore, 787 pp.

Irianto, A. 2005.Patologi Ikan Teleostei. Gajah Mada University Press, Yogyakarta. 243 hal.

Jeney, Z. and Jeney, G., 1995. Recent achievements in studies on diseases of Common carp (Cyprinus carpio). Aquaculture,129 (1-4): 397-420.

Munasir, Z. 2001. Respon Imun terhadap Infeksi Bakteri. Tinjauan Pustaka. Jurnal SariPediatri Vol. 2 (4): 193-197.

Pacha R.E, Ordal E.J. 1967.Histopathology of experimental columnaris disease in young salmon.J Comp Path. 77:419–423.

Plumb, J. 1999. Health Maintanance and Principal Microbial Diseases of Cultured Fishes. Iowa State University, Iowa, 328 pp.

Shamsudin, MN. and Plumb, JA., 1996. Morphological,biochemical and physiological characterization of Flexibacter columnaris isolates from four species of fish. J. Aquat. Anim. Health, 8 (4): 335-339.

Thomas-Jinu, S. and Goodwin, A.E. 2004. Morphological and genetic characteristics of Flavobacteriumcolumnare isolates: correlations with virulence in fish. Journal of Fish Disease, 27: 29-35.

Published
2017-10-12
How to Cite
Jumria, A., Prihartini, N. C., & Alfiyah, A. (2017). IDENTIFICATION AND HISTOPATHOLOGY Flavobacterium columnare ON TILAPIA (Oreochromis niloticus). Samakia : Jurnal Ilmu Perikanan, 8(2), 17-22. https://doi.org/10.5281/jsapi.v8i2.261
Abstract viewed = 800 times
PDF (Bahasa Indonesia) downloaded = 0 times