EFFECT OF Lactobacillus plantarum inoculum 1A-2 AND 1BL-2 silage WASTE OF QUALITY TUNA (Thunnus atlanticus)

Abstract

One effort to the waste treatment is through the process of making fish silage. The main BAL cultures used in research adalah Lactobacillus-2 1BL plantarum and Lactobacillus plantarum 1A-2 obtained from the microbiology laboratory University School of Medicine Brawijaya. Waste tuna obtained from PT. Beeje Food Probolinggo. Treatment applied by varying the addition of lactic acid bacteria inoculum (0%, 0.1%, 0.3%, 0.5% and 1% (v / w)), either as a single inoculum (L. plantarum 1BL- 2 or L. plantarum 1A-2) and as an inoculum mixture (L. 1BL plan-indigo-2 and 1A-2) .Parameter measured as good quality silage is: dry matter (% DM), ash (%), organic matter (% BO), silage temperature (⁰C), the percentage of damage to silage, pH, the amount of BAL (log 10 cfu). From this study showed that the use of Lactobacillus plantarum inoculum 1A-2 and Lactobacillus plantarum 1BL-2 with different variations and concentrations provides good enough influence on the quality of silage as feed for fish. Single inoculum 1A-2 produces a lower pH (3,67- 4,18) and lactic acid content of 0.30 to 0.34 mg mL^-1. In all treatments, the level of damage is quite small (<5%). Loss of dry matter, with the addition of a single inoculum provide a relatively small number of loss is 1BL-2 (0.01 to 3.97%) and 1A-2 (0.31 to 5.18%). Inoculum concentration treatments do not provide a real difference to the quality of silage, making it the smallest concentration at 0.1% v / w which is recommended to be added to the silage-making.