EFFECT OF Lactobacillus plantarum inoculum 1A-2 AND 1BL-2 silage WASTE OF QUALITY TUNA (Thunnus atlanticus)

  • Ramli Ramli Program Studi Pengolahan Hasil Perikanan, Akademi Perikanan Ibrahimy
Keywords: Waste Tuna, Silage, Fish Feed.

Abstract

One effort to the waste treatment is through the process of making fish silage. The main BAL cultures used in research adalah Lactobacillus-2 1BL plantarum and Lactobacillus plantarum 1A-2 obtained from the microbiology laboratory University School of Medicine Brawijaya. Waste tuna obtained from PT. Beeje Food Probolinggo. Treatment applied by varying the addition of lactic acid bacteria inoculum (0%, 0.1%, 0.3%, 0.5% and 1% (v / w)), either as a single inoculum (L. plantarum 1BL- 2 or L. plantarum 1A-2) and as an inoculum mixture (L. 1BL plan-indigo-2 and 1A-2) .Parameter measured as good quality silage is: dry matter (% DM), ash (%), organic matter (% BO), silage temperature (0C), the percentage of damage to silage, pH, the amount of BAL (log 10 cfu). From this study showed that the use of Lactobacillus plantarum inoculum 1A-2 and Lactobacillus plantarum 1BL-2 with different variations and concentrations provides good enough influence on the quality of silage as feed for fish. Single inoculum 1A-2 produces a lower pH (3,67- 4,18) and lactic acid content of 0.30 to 0.34 mg mL-1. In all treatments, the level of damage is quite small (<5%). Loss of dry matter, with the addition of a single inoculum provide a relatively small number of loss is 1BL-2 (0.01 to 3.97%) and 1A-2 (0.31 to 5.18%). Inoculum concentration treatments do not provide a real difference to the quality of silage, making it the smallest concentration at 0.1% v / w which is recommended to be added to the silage-making.

References

Barker, S.B. and W.H. Summerson. 1941. The colorimetric determination of lactic acid in biological material. Journal of Biological Chemistry 138: 535-554.

Bolsen, K.K., G. Ashbell, and J.M. Wilkinnson. 1995. Silage additives in biotechnology. In: Wallace, R.J., and A. Chesson (eds.). Animal Feedsand Animal Feeding. Weinheim: VCH.

Caplice, E., G.F. Fitzgerald. 1999. Food fermentation: role of microorganisms in food production and preservation. International Journal of FoodMicrobiologi 50: 131-149.

Cappuccino, J.G. and N. Sherman. 2001. Microbiology; a Laboratory Manual.6th edition. New York: State University of New York.

Ennahar. S., Y. Cai., and Y. Fujita. 2003. Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Applied and Environmental Microbiology 69 (1): 444-451.

Jay, J.M. 2000. Fermentation and fermented dairy products. In: Modern Food Microbiology. 6th edition. Gaithersburg: Aspen Publishers, Inc.

Johnson, P.N., H.F. Grundy, and A.P. Stanway. 1998. The effect of an inoculant additive on the fermentation characteristics of grass silage and bovine performance. Proceeding of British Society of Animal Science: 144.

Johnson. H.E., R.J. Merry, D.R. Davies, D.B. Kell, M.K. Theodorou, and G.W. Griffith. 2005. Vacuum packing: a model system for laboratory scalesilage fermentations. Journal of Applied Microbiology 98: 106-113.

Kuipers, O.P., G. Buist, and J. Kok. 2000. Current strategies for improving food bacteria. Research in Microbiology 151: 815-822.

McDonald, P., A.R. Henderson, and S.J.E. Heron. 1991. The Biochemistry of Silage. 2nd edition. Centerbury: Chalcombe Publications.

Ohmomo, S., S. Nitisinprasart, and S. Hiranpradit. 2002a. Silage-makingand recent trend of dairy farming in Thailand. JARQ 36: 227-234.

Ohmomo, S., O. Tanaka, H.K. Kitamoto, and Y. Cai. 2002b. Silage and microbial performance, old story but new problems. JARQ 36: 59-71.

Parakatsi, A. 1999. Ilmu Nutrisi Makanan Ternak Ruminansia. Jakarta: UI Press.

Ridwan, R. dan Y. Widyastuti. 2003. Pengawetan Hijauan Makanan Ternakdengan Bakteri Asam Laktat; Manual. Cibinong-Bogor: Pusat Penelitian Bioteknologi-LIPI.

Ridwan, R., S. Ratnakomala, G. Kartina, dan Y. Widyastuti. 2005. Pengaruh penambahan dedak padi dan Lactobacillus plantarum 1BL-2 dalam pembuatan silase ikan (Penisetum purpureum). Jurnal MediaPeternakan-IPB. 28 (3): 117-123. Ridwan. R and Y. Widyastuti, 2001. Membuat silase: upaya mengawetkan dan mempertahankan nilai nutrisi hijauan pakan ternak. Warta Biotek-LIPI 15 (1): 9-14.

Steel, R.G.D. dan J.H. Torrie. 1995. Prinsip dan Prosedur Statistik.Penerjemah: Sumantri, B. Jakarta: PT. Gramedia Pustaka Utama.

Weinberg, Z.G., R.E. Muck, and P.J. Weimer. 2003. The survival of silage inoculant lactic acid bacteria in rumen fluid. Journal of AppliedMicrobiology 94: 1066-1071.

Weinberg, Z.G., R.E. Muck, P.J. Weimer, Y. Chen, and M. Gamburg. 2004. Lactic acid bacteria used in inoculants for silage as probiotics for ruminants. Applied Biochemistry and Biotechnology 118: 1-10.

Widyastuti, Y., S. Ratnakomala, and F. Ekawati. 1998. Bakteri Asam Laktatpada Buah-buahan Tropis. [Laporan

Published
2014-04-27
How to Cite
Ramli, R. (2014). EFFECT OF Lactobacillus plantarum inoculum 1A-2 AND 1BL-2 silage WASTE OF QUALITY TUNA (Thunnus atlanticus). Samakia : Jurnal Ilmu Perikanan, 5(1), 25-30. https://doi.org/10.5281/jsapi.v5i1.213
Abstract viewed = 4 times
PDF (Bahasa Indonesia) downloaded = 0 times