DNA Barcoding Ikan Kepala Timah Dan Betok Berdasarkan Gen COI sebagai Ikan Pioneer di Kolong Pascatambang Timah, Pulau Bangka
Abstract
Tin mining activities in the Bangka Belitung Archipelago Province have led to the formation of pits that have not ideal water quality for aquatic organisms in general. Acidic pH and high metal contamination at certain age under certain causes only a few species are able to live well in these ecosystems. This study aimed to determine the identity of the pioneer fishes in ex-tin mining pit <1 year of Bangka Island through DNA barcoding based on COI gene. The pioneer fish in ex-tin mining pit <1 year were Kepala Timah fish dan Betok fish. Species determination was based on sequences similarity in NCBI and BOLD System showed that Ikan Kepala Timah had similarity with Aplocheilus panchax and its morphology indicated Ikan Kepala Timah was Aplocheilus panchax. The sequences similarity of Betok fish in NCBI and BOLD System showed that Betok fish had similarity with Anabas testudineusi and its morphology showed that Betok fish was Anabas testudineusi. The Kimua 2 Parameters (K-2P) Neighbour-Joining (NJ) clearly confirmed that Kepala Timah fish found in ex-tin mining pit <1 year was Aplocheilus panchax, whereas Betok fish was located outside clade of the group. It indicated that Betok fish was found in ex-tin mining pit <1 year can be expected as a morphotype of Anabas testudineusi that needed further confirmation in other studies, both molecularly and morphologically. The sequence data of COI gene of Betok fish found in ex-tin mining pit <1 year was expected can supported available sequence data of Anabas testudineusi in NCBI and BOLD System.
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References
Ahmad, A.B., Hadiaty, R.K., de Alwis Goonatilake, S., Fernado, M., and Kotagama, O. 2019. Anabas testudineus. The IUCN Red List of Threatened Species 2019: e.T166543A60590563
Aprilia, F.E., Soewondo, A., Widodo., and Toha, A.H.A. 2014. Amplifikasi Gen COI dan 16s rRNA dari Invertebrata Laut Plakobranchus ocellatus. Jurnal Biotropika 2(5): 276-278
Ashraf, M.A., Maah, M.J., and Yusoff, I. 2011. Analysis of physio-chemical parameters and distribution of heavy metals in soil and water of ex-mining area of Bestari Jaya, Peninsular Malaysia. Asian Journal of Chemistry 23(8): 3493-3499
Ashraf, M.A., Maah, M.J., and Yusoff, I. 2012a. Speciation of heavy metals in the sediments of former tin mining catchment. Iranian Journal of Science and Technology 36(A2): 163-180
Ashraf, M.A., Maah, M.J., and Yusoff, I. 2012b. Morphology, geology and water quality assessment of former tin mining catchment. The Scientific World Journal 2012: 1-15
Beck, S.V., Carvalho, G.R., Barlow, A., Ruber, L., Tan, H.H., Nugroho, E., Wowor, D., Nor, S.A.M., Herder, F., Muchlisin, Z.A., and De Bruyn, M. 2017. Plio-pleistocene phylogeography of the Southeast Asian Blue Panchax killifish, Aplocheilus panchax. Plos One 12(7): e0179557
Behera, S., Devi, L.M., Kumar, S., Gogoi, R., Samanta, P., Jomang, O., and Baksi, S. 2015. External morphology and sexual dimorphism of Anabas testudineus in natural environment. International Journal of Science and Nature 6(2): 288-292
Celebi, E.E., and Oncel, M.S. 2016. Determination of acid forming potential of massive sulfide minerals and the tailings situated in lead/zinc mining district of Balya (NW Turkey). Journal of African Earth Sciences 2016(124): 487-496
Daniel, V.N., Chudusu, E.S., Chup, J.A., and Pius, N.D. 2014. Variations of heavy metals in agricultural soils irrigated with tin water in Heipang District of Barkin Ladi, Plateau State, Nigeria. International Journal of Science and Technology 3(5): 255-263
Dopson, M., and Johnson, D.B. 2012. Biodiversity, metabolism and applications of acidophilic sulfur‐metabolizing microorganisms. Environmental Microbiology 14(10): 2620-2631
Furness, A.I. 2015. The evolution of an annual life cycle in killifish: adaptation to ephemeral aquatic environments through embryonic diapause. Biological Reviews 91(3):796-812
Gaikwad, R.W., and Gupta, D.V. 2008. Review on removal of heavy metals from acid mine drainage. Applied Ecology and Environmental Research 6(3): 81-98
Gonzalez-Toril, E., Gomez, F., Malki, M., and Amils, R. 2006. The Isolation and study of acidophilic microorganisms. In Methods in Microbiology Vol. 35: 471-510. Academic Press
Hajibabaei, M., Smith, M.A., Janzen, D.H., Rodriguez, J.J., Whitfield, J.B., and Hebert, P.D.N. 2006. A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes 2006(6): 959-964
Hatar, H., Rahim, S.A., Razi, W.M., and Sahrani, F.K. 2013. Heavy metals content in acid mine drainage at abandoned and active mining area. In AIP Conference Proceedings Vol. 1571, No. 1: 641-646. AIP
Heidel, C., and Tichomirowa, M. 2011. Galena oxidation investigations on oxygen and sulphur isotopes. Isotopes in Environmental and Health Studies 47(2): 169-188
Irawan, R.R., Sumarwan, U., Suharjo, B., and Djohar, S. 2014. Strategic model of tin mining industry in Indonesia (case study of Bangka Belitung Province). International Journal of Business and Management Review 2(3): 48-58
Ivanova, N.V., Zemlak, T.S., Hanner, R.H., and Hebert, P.D. 2007. Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes 7(4): 544-548
Katwate, U., Kumkar, P., Britz, R., Raghavan, R., and Dahanukar, N. 2018. The identity of Aplocheilus andamanicus (Kohler, 1906) (Teleostei: Cyprinodontiformes), an endemic killifish from the Andaman Island, with notes on Odontopsis armata van Hasselt. Zootaxa 4382 (1): 159-174
Kolosova, N., Miller, B., Ralph, S., Ellis, B.E., Douglas, C., Ritland, K., and Bohlmann, J. 2004. Isolation of high-quality RNA from gymnosperm and angiosperm trees. BioTechniques 36(5): 821-824
Kurniawan, A. 2017. Chronosequence effect of post tin mining ponds to metals residu and microecosystem change. Omni-Akuatika 13(1): 60-65
Kurniawan, A. 2019. Diversitas metagenom bakteri di danau pascatambang timah dengan umur berbeda. [Disertasi]. Fakultas Biologi. Universitas Jenderal Soedirman
Kurniawan, A., and Kurniawan, A. 2012. Analisis variasi genetik ikan di kolong pascatambang timah dengan metode elektroforesis. Akuatik 6(2): 6-10
Kurniawan, A., Oedjijono., Tamad., and Sulaeman, U. 2019. The pattern of heavy metals distribution in time chronosequence of ex-tin mining ponds in Bangka Regency, Indonesia. Indonesian Journal of Chemistry 19(1):254-261
Lucena-Aguilar, G., Sanchez-Lopez, A.M., Barberan-Aceituno, C., Carrillo-Avila, J.A., Lopez-Guerrero, J.A., and Aguilar-Quesada, R. 2016. DNA source selection for downstream applications based on DNA quality indicators analysis. Biopreservation and Biobanking 14(4): 264-270
Mejia, E.R., Ospina, J.D., Marquez, M.A., and Morales, A.L. 2009. Oxidation of chalcopyrite (CuFeS2) by Acidithiobacillus ferrooxidans and a mixed culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans like bacterium in shake flasks. In Advanced Materials Research 2009(71-73): 385-388. Trans Tech Publications. Switzerland
Nuryanto, A., Pramono, H., Kusbiyanto, K., Ghifari, M.I., and Andareswari, N. 2018. Barcoding of Fin Clip Samples Revealed High Exploitation of Plectropomus leopardus in Spermonde Archipelago. Biosaintifika: Journal of Biology & Biology Education 10(3): 629-635
Nuryanto, A., and Solihin, D.D. 2006. Variasi Sekuens Gen Mitokondrial Sitokrom C Oksidase I dari Siput Lola (Trochus niloticus). Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal 23(1): 31-37
Parenti, L.R., and Hartel, K.E. 2011. Osteology identifies Fundulus capensis Garman, 1895 as a killifish in the family Fundulidae (Atherinomorpha: Cyprinodontiformes). Copeia 2011(2): 242-250
Prianto, E., Kamal, M. M., Muchsin, I., and Kartamihardja, E.S. 2015. Biologi reproduksi ikan betok (Anabas testudineus) di Paparan Banjiran Lubuk Lampam, Kabupaten Ogan Komering Ilir. BAWAL Widya Riset Perikanan Tangkap 6(3): 137-146
Sedlacek, O., Baciakova, B., and Kratochvil, K. 2014. Evolution of body colouration in killifishes (Cyprinodontiformes: Aplocheilidae, Nothobranchiidae, Rivulidae): is male ornamentation constrained by intersexual genetic correlation?. Zoologischer Anzeiger 253(3): 207-215
Septiyan, R.A., Soendjoto, M.A., and Arifin, Y.F. 2019. Deskripsi lima spesies ikan yang hidup di Kawasan Rawa Desa Malintang, Kecamatan Gambut, Kabupaten Banjar: Survei Pendahuluan. In Prosiding Seminar Nasional Lingkungan Lahan Basah 4(3): 630-634
Slamat, S., Rina, R. K., and Ansyari, P. 2017. Pemulian ikan papuyu (Anabas testudineus) dengan teknik hybridisasi filogenetik meristik dari tiga tipe ekosistem perairan rawa. Intek Akuakultur 1(2): 79-89
Templeton, D.M. 2015. Speciation in metal toxicity and metal-based therapeutics. Toxics 2015(3): 170-186
Trijoko, T., Yudha, D.S., and Eprilurahman, R. (2016). Keanekaragaman jenis ikan di sepanjang Sungai Boyong–Code Propinsi Daerah Istimewa Yogyakarta. Journal of Tropical Biodiversity and Biotechnology 1(1): 21-29
Zhang, Z. 2011. Animal biodiversity: an outline of higher-level classification and survey of taxonomic richness (Addenda 2013). Zootaxa 2011(3148): 1-237
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